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RADIL Introduces Pinworm by PCR Testing
11.19.2009

In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam.  With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results.  RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation.  In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).

Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay.  For more information and pricing, please click here. 

( for more info click here )
MFI2 - The next generation in serology testing
11.19.2009

At this year's National AALAS Meeting in Denver, Colorado, RADIL introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents.  MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available.   By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results.  Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.

For more information regarding MFI2, please visit the Serology section of this site.

( for more info click here )
Stain Method
Purpose
Reference
Alcian Blue, pH 1.0
sulfated mucins
Carson, F Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997, page 120
Alcian Blue, pH 2.5
acid mucins
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Benhold's Congo Red
amyloid
Lillie, R.D., Fullmer, H.M. Histopathologic Technique and Practical Histochemistry, New York, The Blakiston Company, Inc., 1976
Dahl's Alizarin Red S
calcium
Lillie, R.D., Fullmer, H.M. Histopathologic Technique and Practical Histochemistry, New York, The Blakiston Company, Inc., 1976
GMS (Grocott's methenamine silver) Method
fungi
Luna, L.G. Histologic Staining Methods of the AFIP, Third Edition, McGraw-Hill Book Co., New York, 1968
Gomori's Method for Reticulum
reticulum
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Gomori's Modified Iron Stain (Prussian Blue)
oxidized iron
Luna, L.G. Histologic Staining Methods of the AFIP, Third Edition, McGraw-Hill Book Co., New York, 1968
Hall's Method for Bilirubin
bile
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
LFB (Luxol Fast Blue) with Cresyl Violet
myelin, nissel (blue), nuclei (violet)
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Masson's Trichrome
collagen (blue)
Luna, L.G. Histologic Staining Methods of the AFIP, Third Edition, McGraw-Hill Book Co., New York, 1968
May-Grunwald Giemsa Method
microorganisms
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Modified Brown & Hopps
tissue gram stain for bacteria
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Oil Red 0
Neutral lipids
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
PAS (Periodic Acid Schiff)
polysaccharides
Sheehan: Theory and Practice of Histotechnology, 2nd Ed., C.V. Mosby Company, St. Louis, Mo., 1980
PcAg - Ammoniacal Silver
fungi
Journal of Histotechnology, Vol. 11, No.l, pp 19-21, March 1988
Periodic Acid-Methenamine (PAM) Silver Method
basement membranes
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Rhodanine Method
copper
Irons, RD, Chenk, E, Lee, J. Arch. Path. Lab Med. 101:298-301,1977.
Sirius Red with Picric Acid (picrosirius red) Method
collagen
Weber,T K, Cardiovascular Institute, Michael Reese Hosp., Univ. of Chicago Pritzker School of Med., Chicago, III.
Steiner Silver Method
intracellular bacteria
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Toluidine Blue
mast cell granules
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Warthin-Starry Silver Method
spirochetes
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997
Ziehl-Nielsen Method for Acid Fast Bacteria
acid fast bacteria
Sheehan: Theory and Practice of Histotechnology, 2nd Ed., C.V. Mosby Company, St. Louis, Mo., 1980
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