In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam. With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results. RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation. In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).
Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay. For more information and pricing, please click here.
( for more info click here )
At this year's National AALAS Meeting in Denver, Colorado, RADIL
introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents. MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available. By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results. Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.
For more information regarding MFI2, please visit the Serology section of this site.
( for more info click here )Stain Method |
Purpose |
Reference |
Alcian Blue, pH 1.0 |
sulfated mucins |
Carson, F Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997, page 120 |
Alcian Blue, pH 2.5 |
acid mucins |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Benhold's Congo Red |
amyloid |
Lillie, R.D., Fullmer, H.M. Histopathologic Technique and Practical Histochemistry, New York, The Blakiston Company, Inc., 1976 |
Dahl's Alizarin Red S |
calcium |
Lillie, R.D., Fullmer, H.M. Histopathologic Technique and Practical Histochemistry, New York, The Blakiston Company, Inc., 1976 |
GMS (Grocott's methenamine silver) Method |
fungi |
Luna, L.G. Histologic Staining Methods of the AFIP, Third Edition, McGraw-Hill Book Co., New York, 1968 |
Gomori's Method for Reticulum |
reticulum |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Gomori's Modified Iron Stain (Prussian Blue) |
oxidized iron |
Luna, L.G. Histologic Staining Methods of the AFIP, Third Edition, McGraw-Hill Book Co., New York, 1968 |
Hall's Method for Bilirubin |
bile |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
LFB (Luxol Fast Blue) with Cresyl Violet |
myelin, nissel (blue), nuclei (violet) |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Masson's Trichrome |
collagen (blue) |
Luna, L.G. Histologic Staining Methods of the AFIP, Third Edition, McGraw-Hill Book Co., New York, 1968 |
May-Grunwald Giemsa Method |
microorganisms |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Modified Brown & Hopps |
tissue gram stain for bacteria |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Oil Red 0 |
Neutral lipids |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
PAS (Periodic Acid Schiff) |
polysaccharides |
Sheehan: Theory and Practice of Histotechnology, 2nd Ed., C.V. Mosby Company, St. Louis, Mo., 1980 |
PcAg - Ammoniacal Silver |
fungi |
Journal of Histotechnology, Vol. 11, No.l, pp 19-21, March 1988 |
Periodic Acid-Methenamine (PAM) Silver Method |
basement membranes |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Rhodanine Method |
copper |
Irons, RD, Chenk, E, Lee, J. Arch. Path. Lab Med. 101:298-301,1977. |
Sirius Red with Picric Acid (picrosirius red) Method |
collagen |
Weber,T K, Cardiovascular Institute, Michael Reese Hosp., Univ. of Chicago Pritzker School of Med., Chicago, III. |
Steiner Silver Method |
intracellular bacteria |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Toluidine Blue |
mast cell granules |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Warthin-Starry Silver Method |
spirochetes |
Carson, F. Histotechnology: A Self-Instructional Text, 2nd ed., ASCP Press, Chicago, IL, 1997 |
Ziehl-Nielsen Method for Acid Fast Bacteria |
acid fast bacteria |
Sheehan: Theory and Practice of Histotechnology, 2nd Ed., C.V. Mosby Company, St. Louis, Mo., 1980 |