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PROTOZOA OF RODENTS AND RABBITS

COCCIDIA

	Slide 1 is a photo of an unsporulated oocyst of Eimeria magna, a highly pathogenic coccidia of rabbits. Note the characteristic large micropyle.
	Slide 2 is a photo of a sporulated oocyst of Eimeria magna. Note the presence of a large central residular body.
	Slide 3 is a photo of unsporulated oocysts of Eimeria magna and Eimeria perforans, a mildly pathogenic coccidia of rabbits. Note the smaller size and lack of a micropyle on the E. perforans oocyst.
	Slide 4 is a photo of a histologic section of colon from a mouse infected with coccidia. Note that the coccidial forms are present within the colonic epithelial cells. Coccidia as part of their life cycle must live within cells.
	Slide 5 is a photo of a liver from a rabbit infected with hepatic coccidia, Eimeria stiedae. Note the multiple white foci on all liver lobes.
	Slide 6 is a histologic section of a liver from a rabbit infected with a hepatic coccidia. This slide depicts coccidial infection of the bile duct epithelium. Again note the intracellular location.
	Slide 7 is a photo of an unsporulated oocyst of Eimeria stiedae. These oocyts can be found in either the bile or intestinal contents (feces). Note the thin micopyle.
	Slide 8 is a photo of a histologic section of an intestine of a guinea pig infected with Cryptosporidium wrairi. Note the tiny basophilic (blue) coccidia within the brush border of the intestinal epithelial cells.
	Slide 9 is a photo of a histologic section of a kidney from a mouse infected with Klossiella muris. Note the intracellular location of this coccidia within renal epithelial cells.

FLAGELLATES

	Slide 10 is a photo of the cyst and trophozoite form of Spironucleus muris. Note the teardrop shape of the trophozoite and its flagella (there are six anterior and two posterior).
	Slide 11 is a photo of electron micrograph of Spironucleus muris. Again note its shape and flagella.
	Slide 12 is a photo of differential interference contrast (DIC) photo of a Giardia muris trophozoite. Note the two anterior nuclei ("eyespots") which give the organism a "monkey face" appearance.
	Slide 13 is a DIC photo of a Giardia muris trophozoite (8:00). In this photo one anterior nucleus, the two slender axostyles and three of the eight flagella Giardia are evident. Also note the trophozoite of Spironucleus muris (3:00).
	Slide 14 is a DIC photo of the trophozoite of Tritrichomonas muris. Note its lemon shape (Tritrichomonas sp. also have a characteristic undulating membrane and three flagella; these features are not evident on this slide but should be apparent on wet mounts). NOTE: Tritrichomonas sp. do not form true cysts.

SARCODINES (AMOEBAS)

	Slide 15 is a DIC photo of an iodine-stained Entamoeba muris cyst. Note its round shape, vacuoles and eccentric nucleus (Entamoeba cysts may have up to eight nuclei depending on their maturity.
	Slide 16 is a photo of an Entamoeba muris trophozoite. Note its irregular shape and the extension of a pseudopod towards the upper right corner of the slide.

CILIATES

	Slide 17 is a photo of a Balantidium caviae trophozoite. Note its ovoid shape, the large eccentric nucleus, the indentation at one pole and numerous cilia covering the surface of the organism.

NEOSPORANS

	Slide 18 is a photo of a gram stained histologic section of kidney from a rabbit infected with Encephalitozoon cuniculi. Note the gram positive organism-filled pseudocyst within the renal tubular epithelial cells.
	Slide 19 is a photo of a gram stained histologic section of brain from a rabbit infected with Encephalitozoon cuniculi. Note the two pseudocysts full of gram positive organisms.