RADIL STANDARD OPERATING PROCEDURE FOR GASTROINTESTINAL REMOVAL AND FLUSHING
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Step 1. Expose the peritoneal cavity by reflecting the skin and subcutaneous tissue. |
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Step 2. Cut the stomach at the border of the nonglandular and glandular portion. |
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Step 3. Grasp the stomach and cut the duodenum a few centimeters from the pylorus. |
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Step 4. Remove the jejunum by trimming away the mesentery until the entire length is exposed. |
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Step 5. Cut the ileum several centimeters from the cecum to free the jejunum. |
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Step 6. Cut the anterior colon a few centimeters from the cecum and remove the ileum/cecum/colon complex. |
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Step 7. Remove a section of the distal colon. |
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Step 8. Fill a syringe with formalin and place the needle into the lumen of the stomach. Flush out all contents from the stomach and duodenum and place the stomach/duodenum in formalin. |
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Step 9. Place the needle tip in the lumen of the jejunum and grasp the needle with forceps (to prevent the backflow of formalin). Flush all contents out of the jejunum and place the gut rull in formalin. |
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Step 10. Place the tip of the needle in the lumen of the ileum, colon, or through the wall of the cecum and flush all contents out. Place the ileum/cecum/colon in formalin. |
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Step 11. Place the tip of the needle in the distal colon and flush out any fecal pellets. Place the distal colon in formalin. |
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Step 12. Here is an example of what the 4 tissues look like after flushing (stomach at top, jejunum in middle, and cecum and colon at the end). |