RADIL STANDARD OPERATING PROCEDURE FOR COLLECTION OF GUINEA PIG SUBMANDIBULAR SALIVARY GLANDS
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Step 1. Incise and reflect skin from the chin to the cranial thoracic area. |
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Step 2. Remove subcutaneous cervical fat to reveal the bilateral lobes of the thymus. the pointer shows the right lobe. |
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Step 3. Beneath the thymus is the submandibular salivary gland. The pointer shows the right submandibular salivary gland. |
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Step 4. Remove the thymus to expose the parotid salivary gland. The pointer shows the right parotid salivary gland. |
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Step 5. Reflect the parotid salivary gland to expose the submandibular salivary gland, a lymph node, and the thyroid. The pointer shows the reflected parotid salivary gland. |
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Step 6. Note the lymph node (pointer) caudal to the salivary gland. |
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Step 7. Note the right thyroid gland (pointer) cranial and medial to the salivary gland. |
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Step 8. Note the position of the right submandibular salivary gland; the thymus and thyroid have been removed. The left submandibular salivary gland is also identified beneath the intact left lobe of the thymus. |
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Step 9. The right submandibular salivary gland has been removed leaving no glandular tissue on that side of the deep cervical region. |
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Step 10. The thymus, thyroid gland, lymph nodes, and salivary glands have been removed from both sides. |
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Step 11. Note the gross appearance of the thymus (A), parotid salivary gland (B), submandibular salivary gland (C), lymph node (D), and thyroid (E). |
| NOTE 1. If there is a doubt about which tissue is the submandibular salivary gland, always collect all the tissue in cervical region, ensuring that the deep glandular tissue is included. |
NOTE 2. On the ventral surface and attached to the submandibular salivary gland is the round white sublingual gland (not shown in figures). This gland may be either separated from or submitted with the submandibular salivary gland. |