Gene Description

Symbol:  Pln

Gene:  phospholamban

Chromosome:  10

Allele:  Plntm1Egk

Background Strain:  Mixed

Strain Development:  This strain is a targeted mutation in which the Pln gene function has been disrupted. D3 ES cells containing the disrupted Pln gene were injected into C57BL/6 blastocysts. Chimeric offspring were mated to CF-1 mice and carrier offspring were mated together to produce mice homozygous for the Pln knockout allele. This colony has been maintained by sibling mating of homozygous mice.

Other Strain Names:  Phospholamban knockout; PLBKO

Summary of Phenotype

The published phenotype of this mouse model consists of enhanced cardiac myocyte mechanics and Ca²⁺ kinetics which result in significantly higher rates of contraction and relaxation of cardiac myocytes without alteration of the heart rate or left ventricular end-diastolic pressure. This mouse model does not demonstrate any gross or ultrastructural changes in cardiac morphology.

Histologically a myocardial degeneration and/or myofiber mineralization was found in all the mutant mice. Mineralization of brown fat was found in 3 of the mutant mice and a mineralized adrenal gland was found in one mutant mouse. This mineralization of tissues may be related to the genotype, however larger numbers of animals would need to be examined to definitively determine if the lesions found are genotype related.

Summary - Gross Findings

No significant lesions associated with this genotype were found on gross necropsy.

Organ/Body Weights

	CF1 control female 1	CF1 control female 2	CF1 control male 1	CF1 control male 2
adrenal gland(s)	.0113	.0120	.0075	.0107
body weight	21.98	22.62	31.07	28.29
brain	.3876	.4387	.4416	.4509
heart	.1052	.1270	.1754	.1628
kidney	.1405	.1546	.2892	.3229
liver	1.1794	1.1328	2.0411	1.794
ovary	.0056	.0072
spleen	.0530	.0783	.0810	.0924
testicle			.0986	.0992
	Mutant male 1	Mutant male 2	Mutant male 3	Mutant male 4
adrenal gland(s)	0.0034	0.0030	0.0032	0.0032
body weight	26.82	24.83	25.35	26.54
brain	0.4401	0.4220	0.4293	0.4242
heart	0.1784	0.2110	0.1699	0.1802
kidney	0.2290	0.2265	0.2055	0.2202
liver	1.5121	1.0441	1.4029	1.4434
ovary
spleen	0.0529	0.0673	0.0572	0.0477
testicle	0.0781	0.0605	0.0780	0.0788
	Mutant female 1	Mutant female 2	Mutant female 3	Mutant female 4
adrenal gland(s)	.0048	.0077	.0052	.0048
body weight	19.23	22.64	22.75	22.99
brain	.4056	0.4289	.4383	.4214
heart	.1236	.1375	.1363	.1503
kidney	.1264	0.1452	.1434	.1602
liver	.9771	1.0017	1.1219	1.1606
ovary	.0049	.0041	.0074	.0098
spleen	.0404	.0407	.0476	.0470
testicle
	129/sv control female 1	129/sv control female 2	129/sv control male 1	129/sv control male 2
adrenal gland(s)	.0066	.0069	.0049	.0037
body weight	17.7	17.2	20.9	21.3
brain	.3919	.3670	.3702	.3925
heart	.0945	.1057	.1518	.1667
kidney	.1096	.1046	.1675	.1596
liver	.8987	.8392	1.2222	1.0928
ovary	.0085	.0081
spleen	.0544	.0520	.0672	.0577
testes			.0614	.0754

Summary - Histology

Myocardial degeneration or myofiber mineralization was found in all of the mutant mice. 3/4 mutant males and 4/4 mutant females had focal to multifocal myocardial degneration. 4/4 males and 3/4 females had focal to multifocal areas of myocardial mineralization. Mutant female 3 had unilateral diffuse mineralization of the adrenal gland and multifocal mineralization of the brown fat. Mutant male 4 and mutant female 2 had focal mineralizaion of the brown fat. This mineralization of tissues may be related to the genotype, however larger numbers of animals would need to be examined to definitively determine if the lesions found are genotype related.

Phenotypic Abnormalities - Histology
STOCK Pln^tm1Egk/Hsd (MMRRC:0027-H/M)

Organ	Lesion	Sex	Age	# Affected	Image(s)
adrenal gland(s)	diffuse mineralization and fibrosis	F	8W	1/4
heart	mild focal vasculitis	M	8w	1/4
heart	focal/multifocal myocardial degeneration	M/F	8W	7/8
heart	focal/multifocal myocardial mineralization	M/F	8W	7/8
brown fat	focal/multifocal areas of mineralization	M/F	8W	3/8

Click on lesion for full description. Click on image for larger view.

Complete Data - Histology

(Click on lesion for full description.)

	CF1 control female 1	CF1 control female 2	CF1 control male 1	CF1 control male 2
adrenal gland(s)
X-zone regression	+	+	-	-
diffuse mineralization and fibrosis	-	-	-	-
aorta
no lesions	-	-	-	-
brown fat
focal/multifocal areas of mineralization	-	-	-	-
bulbourethral gland
no lesions			-	-
cecum
mucosal lymphoid hyperplasia	+	+	+	+
cerebellum
no lesions	-	-	-	-
cerebrum
no lesions	-	-	-	-
cervix
no lesions	-	-
clitoral gland
no lesions	-	-
coagulating gland
no lesions			-	-
costrochondral junction
no lesions	-	-	-	-
diaphragm
no lesions	-	-	-	-
distal colon
no lesions	-	-	-	-
duodenum
no lesions	-	-	-	-
epididymis
no lesions			-	-
exorbital gland
mild multifocal interstitial lymphoid infiltrates	-	-	+	+
mild multifocal periductal lymphoid infiltrates	-	-	+	+
eye
no pigment present	+	+	+	+
mild focal corneal thickening	-	-	-	-
femoral bone marrow
no lesions	-	-	-	-
femur
no lesions	-	-	-	-
Harderian gland
no lesions	-	-	-	-
heart
focal subepicardial mineralization	-	-	-	-
mild focal vasculitis	-	-	-	-
focal/multifocal myocardial mineralization	-	-	-	-
focal/multifocal myocardial degeneration	-	-	-	-
ileum
segmented filamentous bacteria	+	+	+	+
incisor tooth
no lesions	-	-	-	-
inner ear
no lesions	-	-	-	-
jejunum
segmented filamentous bacteria	+	+	+	+
mucosal lymphoid hyperplasia	+	+	+	+
kidneys
focal acute tubular necrosis	-	-	-	-
tubular regeneration	-	-	-	-
focal nephrocalcinosis	-	+	-	-
lacrimal gland(s)
no lesions	-	-	-	-
liver
microgranulomas	+	+	+	-
hematopoiesis	+	+	+	+
periportal foreign body granuloma	-	-	-	+
occasional mitotic figure	-	-	-	-
lung
focal epidermal inclusion cyst	-	-	-	-
subpleural alveolar histiocytosis	-	-	-	-
alveolar histiocytosis	-	-	+	-
mild focal alveolar mineralization	-	-	-	-
mammary gland
no lesions	-	-	-	-
mandibular lymph node(s)
no lesions	-	-	-	-
mesenteric lymph node
mild follicular hyperplasia	-	-	-	-
molar tooth
no lesions	-	-	-	-
nasal cavity
no lesions	-	-	-	-
ovary
no lesions	-	-
pancreas
occasional degenerating acini	+	-	-	-
cytologic alteration present	-	-	-	+
parathyroid gland
no lesions
parotid salivary glands
occasional foci of apoptosis	-	+	-	-
foci of apoptosis in acini	-	-	-	-
pituitary gland
no lesions	-	-	-	-
preputial glands
no lesions			-	-
prostate gland
no lesions			-	-
proximal colon
no lesions	-	-	-	-
quadriceps muscle
no lesions	-	-	-	-
sciatic nerve
no lesions	-	-	-	-
skin
focal follicular cyst	-	-	-	-
focal granuloma	-	-	-	-
spinal cord
focal epithelial iclusion cyst	-	-	-	+
spleen
hematopoiesis	+	+	+	+
mild follicular hyperplasia	-	-	-	-
granulopoiesis	-	+	-	+
stifle
no lesions	-	-	-	-
stomach
focal cyst	-	+	-	-
mild focal epithelial hyperplasia	-	-	-	-
focal keratinic cyst	-	-	-	-
sublingual salivary gland
no lesions	-	-	-	-
submand. salivary glands
periductular lymphoid infiltrate	-	-	-	-
periductular lymphoid aggregates	-	-	-	-
testes
no lesions			-	-
thymus
marked focal atrophy	-	-	-	-
mild follicular atrophy	-	-	-	+
thyroid gland
focal ectopic thymic tissue	-	+	-	-
tongue
focal granuloma	+	-	-	-
mild focal neutrophilic infiltration	-	-	-	-
trachea
no lesions	-	-	-	-
urethra
coagulum present	-	-	-	+
urinary bladder
coagulum present	-	-	-	+
uterus
no lesions	-	-
vagina
no lesions	-	-
vesicular gland
mild focal mucosal hyperplasia			-	-
	Mutant male 1	Mutant male 2	Mutant male 3	Mutant male 4
adrenal gland(s)
X-zone regression	-	-	-	-
diffuse mineralization and fibrosis	-	-	-	-
aorta
no lesions	-	-	-	-
brown fat
focal/multifocal areas of mineralization	-	-	-	+
bulbourethral gland
no lesions	-	-	-	-
cecum
mucosal lymphoid hyperplasia	+	-	-	-
cerebellum
no lesions	-	-	-	-
cerebrum
no lesions	-	-	-	-
cervix
no lesions
clitoral gland
no lesions
coagulating gland
no lesions	-	-	-	-
costrochondral junction
no lesions	-	-	-	-
diaphragm
no lesions	-	-	-	-
distal colon
no lesions	-	-	-	-
duodenum
no lesions	-	-	-	-
epididymis
no lesions	-	-	-	-
exorbital gland
mild multifocal interstitial lymphoid infiltrates	+	-	-	-
mild multifocal periductal lymphoid infiltrates	-	-	+	+
eye
no pigment present	-	-	-	-
mild focal corneal thickening	-	-	-	-
femoral bone marrow
no lesions	-	-	-	-
femur
no lesions	-	-	-	-
Harderian gland
no lesions	-	-	-	-
heart
focal subepicardial mineralization	-	-	-	-
mild focal vasculitis	+	-	-	-
focal/multifocal myocardial mineralization	+	+	+	+
focal/multifocal myocardial degeneration	+	+	+	-
ileum
segmented filamentous bacteria	+	+	+	+
incisor tooth
no lesions	-	-	-	-
inner ear
no lesions	-	-	-	-
jejunum
segmented filamentous bacteria	+	+	+	+
mucosal lymphoid hyperplasia	+	+	+	-
kidneys
focal acute tubular necrosis	-	-	-	-
tubular regeneration	-	-	-	-
focal nephrocalcinosis	-	-	-	-
lacrimal gland(s)
no lesions	-	-	-	-
liver
microgranulomas	-	-	-	-
hematopoiesis	-	+	-	-
periportal foreign body granuloma	-	-	-	-
occasional mitotic figure	-	+	-	-
lung
focal epidermal inclusion cyst	-	-	-	-
subpleural alveolar histiocytosis	-	-	-	-
alveolar histiocytosis	-	+	-	-
mild focal alveolar mineralization	-	-	-	-
mammary gland
no lesions	-	-	-	-
mandibular lymph node(s)
no lesions	-	-	-	-
mesenteric lymph node
mild follicular hyperplasia	-	-	-	-
molar tooth
no lesions	-	-	-	-
nasal cavity
no lesions	-	-	-	-
ovary
no lesions
pancreas
occasional degenerating acini	-	-	+	-
cytologic alteration present	+	+	-	+
parathyroid gland
no lesions				-
parotid salivary glands
occasional foci of apoptosis	-	-	-	-
foci of apoptosis in acini	+	-	-	-
pituitary gland
no lesions	-	-	-	-
preputial glands
no lesions	-	-	-	-
prostate gland
no lesions	-	-	-	-
proximal colon
no lesions	-	-	-	-
quadriceps muscle
no lesions	-	-	-	-
sciatic nerve
no lesions	-	-	-	-
skin
focal follicular cyst	-	-	-	-
focal granuloma	-	-	-	-
spinal cord
focal epithelial iclusion cyst	-	-	-	-
spleen
hematopoiesis	+	+	-	+
mild follicular hyperplasia	-	-	-	-
granulopoiesis	-	-	-	-
stifle
no lesions	-	-	-	-
stomach
focal cyst	-	-	-	-
mild focal epithelial hyperplasia	+	-	-	-
focal keratinic cyst	-	-	+	-
sublingual salivary gland
no lesions	-	-	-	-
submand. salivary glands
periductular lymphoid infiltrate	-	-	-	-
periductular lymphoid aggregates	-	-	-	-
testes
no lesions	-	-	-	-
thymus
marked focal atrophy	-	-	-	-
mild follicular atrophy	-	-	-	-
thyroid gland
focal ectopic thymic tissue	+	-	-	-
tongue
focal granuloma	-	-	-	-
mild focal neutrophilic infiltration	-	-	-	-
trachea
no lesions	-	-	-	-
urethra
coagulum present	+	+	+	+
urinary bladder
coagulum present	-	-	+	+
uterus
no lesions
vagina
no lesions
vesicular gland
mild focal mucosal hyperplasia	-	+	-	-
	Mutant female 1	Mutant female 2	Mutant female 3	Mutant female 4
adrenal gland(s)
X-zone regression	+	-	-	+
diffuse mineralization and fibrosis	-	-	+	-
aorta
no lesions	-	-	-	-
brown fat
focal/multifocal areas of mineralization	-	+	+	-
bulbourethral gland
no lesions
cecum
mucosal lymphoid hyperplasia	-	-	-	-
cerebellum
no lesions	-	-	-	-
cerebrum
no lesions	-	-	-	-
cervix
no lesions	-	-	-	-
clitoral gland
no lesions	-	-	-	-
coagulating gland
no lesions
costrochondral junction
no lesions	-	-	-	-
diaphragm
no lesions	-	-	-	-
distal colon
no lesions	-	-	-	-
duodenum
no lesions	-	-	-	-
epididymis
no lesions
exorbital gland
mild multifocal interstitial lymphoid infiltrates	-	-	-	+
mild multifocal periductal lymphoid infiltrates	-	+	+	-
eye
no pigment present	-	-	-	-
mild focal corneal thickening	-	-	-	+
femoral bone marrow
no lesions	-	-	-	-
femur
no lesions	-	-	-	-
Harderian gland
no lesions	-	-	-	-
heart
focal subepicardial mineralization	-	-	-	+
mild focal vasculitis	-	-	-	-
focal/multifocal myocardial mineralization	-	+	+	+
focal/multifocal myocardial degeneration	+	+	+	+
ileum
segmented filamentous bacteria	+	+	+	+
incisor tooth
no lesions	-	-	-	-
inner ear
no lesions	-	-	-	-
jejunum
segmented filamentous bacteria	+	+	+	+
mucosal lymphoid hyperplasia	-	+	+	+
kidneys
focal acute tubular necrosis	-	-	-	-
tubular regeneration	-	-	-	-
focal nephrocalcinosis	-	-	-	-
lacrimal gland(s)
no lesions	-	-	-	-
liver
microgranulomas	-	-	-	-
hematopoiesis	-	+	-	-
periportal foreign body granuloma	-	-	-	-
occasional mitotic figure	-	-	-	-
lung
focal epidermal inclusion cyst	-	+	-	-
subpleural alveolar histiocytosis	-	-	-	-
alveolar histiocytosis	-	-	-	-
mild focal alveolar mineralization	+	-	-	-
mammary gland
no lesions	-	-	-	-
mandibular lymph node(s)
no lesions	-	-	-	-
mesenteric lymph node
mild follicular hyperplasia	-	-	+	+
molar tooth
no lesions	-	-	-	-
nasal cavity
no lesions	-	-	-	-
ovary
no lesions	-	-	-	-
pancreas
occasional degenerating acini	-	-	-	-
cytologic alteration present	+	-	-	+
parathyroid gland
no lesions	-	-
parotid salivary glands
occasional foci of apoptosis	-	-	-	-
foci of apoptosis in acini	+	-	-	-
pituitary gland
no lesions	-	-	-	-
preputial glands
no lesions
prostate gland
no lesions
proximal colon
no lesions	-	-	-	-
quadriceps muscle
no lesions	-	-	-	-
sciatic nerve
no lesions	-	-	-	-
skin
focal follicular cyst	+	-	-	-
focal granuloma	+	-	-	-
spinal cord
focal epithelial iclusion cyst	-	-	-	-
spleen
hematopoiesis	+	+	+	+
mild follicular hyperplasia	-	-	-	-
granulopoiesis	-	-	-	-
stifle
no lesions	-	-	-	-
stomach
focal cyst	-	-	-	-
mild focal epithelial hyperplasia	-	-	-	-
focal keratinic cyst	-	-	-	-
sublingual salivary gland
no lesions		-	-	-
submand. salivary glands
periductular lymphoid infiltrate	-	-	+	-
periductular lymphoid aggregates	-	-	-	+
testes
no lesions
thymus
marked focal atrophy	-	-	-	-
mild follicular atrophy	-	-	-	-
thyroid gland
focal ectopic thymic tissue	-	-	-	-
tongue
focal granuloma	-	-	-	-
mild focal neutrophilic infiltration	-	-	-	-
trachea
no lesions	-	-	-	-
urethra
coagulum present	-	-	-	-
urinary bladder
coagulum present	-	-	-	-
uterus
no lesions	-	-	-	-
vagina
no lesions	-	-	-	-
vesicular gland
mild focal mucosal hyperplasia
	129/sv control female 1	129/sv control female 2	129/sv control male 1	129/sv control male 2
adrenal gland(s)
X-zone regression	+	-	-	-
diffuse mineralization and fibrosis	-	-	-	-
aorta
no lesions	-	-	-	-
brown fat
focal/multifocal areas of mineralization	-	-	-	-
bulbourethral gland
no lesions			-	-
cecum
mucosal lymphoid hyperplasia	-	+	+	-
cerebellum
no lesions	-	-	-	-
cerebrum
no lesions	-	-	-	-
cervix
no lesions	-	-
clitoral gland
no lesions	-	-
coagulating gland
no lesions			-	-
costrochondral junction
no lesions	-	-	-	-
diaphragm
no lesions	-	-	-	-
distal colon
no lesions	-	-	-	-
duodenum
no lesions	-	-	-	-
epididymis
no lesions			-	-
exorbital gland
mild multifocal interstitial lymphoid infiltrates	-	-	-	-
mild multifocal periductal lymphoid infiltrates	-	-	-	-
eye
no pigment present	+	+	+	+
mild focal corneal thickening	-	-	-	-
femoral bone marrow
no lesions	-	-	-	-
femur
no lesions	-	-	-	-
Harderian gland
no lesions	-	-	-	-
heart
focal subepicardial mineralization	-	-	-	-
mild focal vasculitis	-	-	-	-
focal/multifocal myocardial mineralization	-	-	-	-
focal/multifocal myocardial degeneration	-	-	-	-
ileum
segmented filamentous bacteria	+	+	+	+
incisor tooth
no lesions	-	-	-	-
inner ear
no lesions	-	-	-	-
jejunum
segmented filamentous bacteria	+	+	+	+
mucosal lymphoid hyperplasia	+	+	+	+
kidneys
focal acute tubular necrosis	-	+	-	-
tubular regeneration	-	-	+	-
focal nephrocalcinosis	-	-	-	-
lacrimal gland(s)
no lesions	-	-	-	-
liver
microgranulomas	+	+	+	+
hematopoiesis	+	-	+	+
periportal foreign body granuloma	-	-	-	-
occasional mitotic figure	-	-	+	-
lung
focal epidermal inclusion cyst	-	-	-	-
subpleural alveolar histiocytosis	-	-	+	-
alveolar histiocytosis	-	-	-	-
mild focal alveolar mineralization	-	-	-	-
mammary gland
no lesions	-	-	-	-
mandibular lymph node(s)
no lesions	-	-	-	-
mesenteric lymph node
mild follicular hyperplasia	-	-		+
molar tooth
no lesions	-	-	-	-
nasal cavity
no lesions	-	-	-	-
ovary
no lesions	-	-
pancreas
occasional degenerating acini	-	-	-	-
cytologic alteration present	+	+	-	-
parathyroid gland
no lesions
parotid salivary glands
occasional foci of apoptosis	-	-	-	-
foci of apoptosis in acini	-	-	-	-
pituitary gland
no lesions	-	-	-	-
preputial glands
no lesions			-	-
prostate gland
no lesions			-	-
proximal colon
no lesions	-	-	-	-
quadriceps muscle
no lesions	-	-	-	-
sciatic nerve
no lesions	-	-	-	-
skin
focal follicular cyst	-	-	-	-
focal granuloma	-	-	-	-
spinal cord
focal epithelial iclusion cyst	-	-	-	-
spleen
hematopoiesis	+	+	+	+
mild follicular hyperplasia	+	-	-	-
granulopoiesis	-	-	-	-
stifle
no lesions	-	-	-	-
stomach
focal cyst	-	-	-	-
mild focal epithelial hyperplasia	-	-	-	-
focal keratinic cyst	-	-	-	-
sublingual salivary gland
no lesions	-	-	-	-
submand. salivary glands
periductular lymphoid infiltrate	-	-	-	-
periductular lymphoid aggregates	-	-	-	-
testes
no lesions			-	-
thymus
marked focal atrophy	+	-	-	-
mild follicular atrophy	-	-	-	-
thyroid gland
focal ectopic thymic tissue	-	-	-	-
tongue
focal granuloma	-	-	-	-
mild focal neutrophilic infiltration	-	+	-	-
trachea
no lesions	-	-	-	-
urethra
coagulum present	-	-	+	+
urinary bladder
coagulum present	-	-	+	+
uterus
no lesions	-	-
vagina
no lesions	-	-
vesicular gland
mild focal mucosal hyperplasia			-	-

Summary - Behavioral Testing

A series of behavioral tests were performed on all 16 mice. All 16 mice responded within normal limits. No differences were observed between mutant mice and their controls.

Complete Data - Behavioral Testing

Reflex Testing

	CF1 control female 1	CF1 control female 2	CF1 control male 1	CF1 control male 2
visible	wnl	wnl	wnl	wnl
spontaneous behavior	wnl	wnl	wnl	wnl
reaching reflex	wnl	wnl	wnl	wnl
righting reflex	wnl	wnl	wnl	wnl
trunk curl	wnl	wnl	wnl	wnl
negative geotaxis	wnl	wnl	wnl	wnl
hanging wire grip test	wnl	wnl	wnl	wnl
preyer reflex	wnl	wnl	wnl	wnl
pinna reflex	wnl	wnl	wnl	wnl
vibrissae reflex	wnl	wnl	wnl	wnl
pupillary light reflex	NA	NA	NA	NA
	Mutant male 1	Mutant male 2	Mutant male 3	Mutant male 4
visible	wnl	wnl	wnl	wnl
spontaneous behavior	wnl	wnl	wnl	wnl
reaching reflex	wnl	wnl	wnl	wnl
righting reflex	wnl	wnl	wnl	wnl
trunk curl	wnl	wnl	wnl	wnl
negative geotaxis	wnl	wnl	wnl	wnl
hanging wire grip test	wnl	wnl	wnl	wnl
preyer reflex	wnl	wnl	wnl	wnl
pinna reflex	wnl	wnl	wnl	wnl
vibrissae reflex	wnl	wnl	wnl	wnl
pupillary light reflex	NA	NA	NA	NA
	Mutant female 1	Mutant female 2	Mutant female 3	Mutant female 4
visible	wnl	wnl	wnl	wnl
spontaneous behavior	wnl	wnl	wnl	wnl
reaching reflex	wnl	wnl	wnl	wnl
righting reflex	wnl	wnl	wnl	wnl
trunk curl	wnl	wnl	wnl	wnl
negative geotaxis	wnl	wnl	wnl	wnl
hanging wire grip test	wnl	wnl	wnl	wnl
preyer reflex	wnl	wnl	wnl	wnl
pinna reflex	wnl	wnl	wnl	wnl
vibrissae reflex	wnl	wnl	wnl	wnl
pupillary light reflex	NA	NA	NA	NA
	129/sv control female 1	129/sv control female 2	129/sv control male 1	129/sv control male 2
visible	wnl	wnl	wnl	wnl
spontaneous behavior	wnl	wnl	wnl	wnl
reaching reflex	wnl	wnl	wnl	wnl
righting reflex	wnl	wnl	wnl	wnl
trunk curl	wnl	wnl	wnl	wnl
negative geotaxis	wnl	wnl	wnl	wnl
hanging wire grip test	wnl	wnl	wnl	wnl
preyer reflex	wnl	wnl	wnl	wnl
pinna reflex	wnl	wnl	wnl	wnl
vibrissae reflex	wnl	wnl	wnl	wnl
pupillary light reflex	NA	NA	NA	NA

 

Open Field Locomotion

Total Activity

	CF1 control female 1	CF1 control female 2	CF1 control male 1	CF1 control male 2
Ambulatory Distance
Sum min 1-60	19407.87	31930.34	20163.89	17295.84
Average min 1-60	323.46	532.17	336.06	288.26
Sum min 11-60	13723.89	22788.83	14367.79	11702.49
Average min 11-60	274.48	455.78	287.36	234.05
Ambulatory Time
Sum min 1-60	762.10	1033.40	761.15	631.60
Average min 1-60	12.70	17.22	12.69	10.53
Sum min 11-60	537	749.35	531.85	414.30
Average min 11-60	10.74	14.99	10.64	8.29
Ambulatory Counts
Sum min 1-60	8094	14164	8612	7555
Average min 1-60	134.90	236.07	143.53	125.92
Sum min 11-60	5662	10015	6053	5038
Average min 11-60	113.24	200.30	121.06	100.76
Ambulatory Episodes
Sum min 1-60	808	1052	804	685
Average min 1-60	13.47	17.53	13.40	11.42
Sum min 11-60	600	825	577	480
Average min 11-60	12	16.5	11.54	9.60
Stereotypical Time
Sum min 1-60	420.40	378.90	496.30	422.70
Average min 1-60	7	6.32	8.27	7.05
Sum min 11-60	330.05	302.10	390.80	323.35
Average min 11-60	6.60	6.04	7.82	6.47
Stereotypical Counts
Sum min 1-60	5048	4762	5995	5103
Average min 1-60	84.13	79.37	99.92	85.05
Sum min 11-60	3957	3769	4723	3902
Average min 11-60	79.14	75.38	94.46	78.04
Rest Time
Sum min 1-60	2244.10	1893.35	2272.15	2482.90
Average min 1-60	37.40	31.56	37.87	41.38
Sum min 11-60	1931.05	1639.70	1952.10	2148.35
Average min 11-60	38.62	32.79	39.04	42.97
Vertical Counts
Sum min 1-60	756.80	897.40	963.35	898.15
Average min 1-60	12.61	14.96	16.06	14.97
Sum min 11-60	647.85	779.10	828.25	783.05
Average min 11-60	12.96	15.58	16.57	15.66
Vertical Time
Sum min 1-60	1314	2155	1710	1910
Average min 1-60	21.90	35.92	28.50	31.83
Sum min 11-60	1104	1822	1425	1667
Average min 11-60	22.08	36.44	28.50	33.34
Clockwise Rotation
Sum min 1-60	60	142	108	49
Average min 1-60	1	2.37	1.80	.82
Sum min 11-60	46	101	79	33
Average min 11-60	.92	2.02	1.58	.66
Counter Clockwise Rotation
Sum min 1-60	126	139	76	134
Average min 1-60	2.10	2.32	1.27	2.23
Sum min 11-60	88	99	48	94
Average min 11-60	1.76	1.98	.96	1.88
Jump Counts
Sum min 1-60	173.4	294.35	70.40	62.80
Average min 1-60	2.89	4.91	1.17	1.05
Sum min 11-60	141.9	248.85	65.25	54
Average min 11-60	2.84	4.98	1.31	1.08
Jump Time
Sum min 1-60	741	1784	507	540
Average min 1-60	12.35	29.73	8.45	9
Sum min 11-60	6.13	1480	463	456
Average min 11-60	12.26	29.60	9.26	9.12
Average Velocity
Sum min 1-60	NA	NA	NA	NA
Average min 1-60	32.69	37.35	30.60	33.97
Sum min 11-60	NA
Average min 11-60	33.22	37.13	31.12	35.64
	Mutant male 1	Mutant male 2	Mutant male 3	Mutant male 4
Ambulatory Distance
Sum min 1-60	11137.38	17198.25	11980.45	16581.08
Average min 1-60	185.62	286.64	199.67	276.35
Sum min 11-60	5433.21	10425.01	6235.07	9902.46
Average min 11-60	108.66	208.50	124.70	198.05
Ambulatory Time
Sum min 1-60	439.60	709.95	443.25	683.80
Average min 1-60	7.33	11.83	7.39	11.40
Sum min 11-60	210.25	431.30	224.45	402.50
Average min 11-60	4.21	8.63	4.50	8.05
Ambulatory Counts
Sum min 1-60	4699	7215	4698	6711
Average min 1-60	78.32	120.25	78.30	111.85
Sum min 11-60	2121	4263	2353	3855
Average min 11-60	42.42	85.26	47.06	77.10
Ambulatory Episodes
Sum min 1-60	451	598	450	719
Average min 1-60	7.52	9.97	7.50	11.98
Sum min 11-60	260	412	261	491
Average min 11-60	5.20	8.24	5.22	9.82
Stereotypical Time
Sum min 1-60	330.10	352.80	329.50	425.35
Average min 1-60	5.50	5.88	5.49	7.09
Sum min 11-60	245.30	283.75	248.95	346.10
Average min 11-60	4.91	5.68	4.98	6.92
Stereotypical Counts
Sum min 1-60	3651	3945	3699	4780
Average min 1-60	60.85	65.75	61.65	79.67
Sum min 11-60	2691	3143	2755	3859
Average min 11-60	53.82	62.86	55.10	77.18
Rest Time
Sum min 1-60	2825.10	2531.20	2824	2487.90
Average min 1-60	47.09	42.19	47.07	41.47
Sum min 11-60	2481.80	2219.45	2463.60	2188.90
Average min 11-60	49.64	44.40	49.27	43.78
Vertical Counts
Sum min 1-60	332.15	353.30	436.80	379.10
Average min 1-60	5.54	5.89	7.28	6.32
Sum min 11-60	236.60	281.40	313.65	319.30
Average min 11-60	5.27	5.63	6.27	6.39
Vertical Time
Sum min 1-60	858	562	818	662
Average min 1-60	14.30	9.37	13.63	11.03
Sum min 11-60	645	410	581	511
Average min 11-60	12.90	8.20	11.62	10.22
Clockwise Rotation
Sum min 1-60	56	88	58	62
Average min 1-60	.93	1.47	.97	1.03
Sum min 11-60	27	55	35	47
Average min 11-60	.54	1.10	.70	.94
Counter Clockwise Rotation
Sum min 1-60	52	70	52	109
Average min 1-60	.87	1.17	.87	1.82
Sum min 11-60	24	44	26	64
Average min 11-60	.48	.88	.52	1.28
Jump Counts
Sum min 1-60	5.20	6.05	3.25	2.95
Average min 1-60	.09	.10	.05	.05
Sum min 11-60	2.65	5.50	3	2.50
Average min 11-60	.05	.11	.06	.05
Jump Time
Sum min 1-60	66	76	42	45
Average min 1-60	1.10	1.27	.84	.75
Sum min 11-60	32	68	38	37
Average min 11-60	.64	1.36	.76	.74
Average Velocity
Sum min 1-60	NA	NA	NA	NA
Average min 1-60	29.68	26.65	29.81	29.17
Sum min 11-60
Average min 11-60	29.40	26.80	30.39	29.66
	Mutant female 1	Mutant female 2	Mutant female 3	Mutant female 4
Ambulatory Distance
Sum min 1-60	19016.89	11675.32	17094.22	22085.66
Average min 1-60	316.95	194.59	284.90	368.09
Sum min 11-60	12829.21	8012.78	11175.49	15624.74
Average min 11-60	256.58	160.26	223.51	312.49
Ambulatory Time
Sum min 1-60	847.20	540.20	805.80	917.40
Average min 1-60	14.12	9	13.43	15.29
Sum min 11-60	580.40	367.50	527.20	654.80
Average min 11-60	11.61	7.35	10.54	13.10
Ambulatory Counts
Sum min 1-60	7573	4355	6817	9476
Average min 1-60	126.22	72.58	113.62	157.93
Sum min 11-60	4968	2976	4351	6651
Average min 11-60	99.36	59.52	87.02	133.02
Ambulatory Episodes
Sum min 1-60	813	516	665	839
Average min 1-60	13.55	8.60	11.08	13.98
Sum min 11-60	600	362	470	657
Average min 11-60	12	7.24	9.40	13.14
Stereotypical Time
Sum min 1-60	556.90	578.60	567.50	575.50
Average min 1-60	9.28	9.64	9.46	9.59
Sum min 11-60	450.80	431.60	446.80	461.80
Average min 11-60	9.02	8.63	8.94	9.24
Stereotypical Counts
Sum min 1-60	3539	3482	3532	3739
Average min 1-60	58.98	58.03	58.87	62.32
Sum min 11-60	2846	2570	2744	2969
Average min 11-60	56.92	51.40	54.88	59.38
Rest Time
Sum min 1-60	2153.80	2479.40	2219.60	2074.50
Average min 1-60	35.90	41.32	36.99	34.58
Sum min 11-60	1874.70	2139.50	1959.40	1793.10
Average min 11-60	37.49	42.79	39.19	35.86
Vertical Counts
Sum min 1-60	436.70	220.70	440.80	752.80
Average min 1-60	7.28	3.68	7.35	12.55
Sum min 11-60	319.40	183.50	385.20	638.40
Average min 11-60	6.39	3.67	7.70	12.77
Vertical Time
Sum min 1-60	714	387	736	961
Average min 1-60	11.90	6.45	12.27	16.02
Sum min 11-60	519	307	609	781
Average min 11-60	10.38	6.14	12.18	15.62
Clockwise Rotation
Sum min 1-60	70	24	65	109
Average min 1-60	1.17	.40	1.08	1.82
Sum min 11-60	57	20	37	83
Average min 11-60	1.14	.40	.74	1.66
Counter Clockwise Rotation
Sum min 1-60	114	80	92	107
Average min 1-60	1.90	1.33	1.53	1.78
Sum min 11-60	70	56	66	73
Average min 11-60	1.40	1.12	1.32	1.46
Jump Counts
Sum min 1-60	42.10	1.80	6.70	32.20
Average min 1-60	.70	.03	.11	.54
Sum min 11-60	34.10	1.40	6.20	29.90
Average min 11-60	.68	.03	.12	.60
Jump Time
Sum min 1-60	206	16	49	211
Average min 1-60	3.43	.27	.82	3.52
Sum min 11-60	166	12	45	191
Average min 11-60	3.32	.24	.90	3.82
Average Velocity
Sum min 1-60	NA	NA	NA	NA
Average min 1-60	25.19	22.77	23.12	27.77
Sum min 11-60
Average min 11-60	24.80	22.38	23.37	28.16
	129/sv control female 1	129/sv control female 2	129/sv control male 1	129/sv control male 2
Ambulatory Distance
Sum min 1-60	11774.56	10472.31	8042.63	12907.46
Average min 1-60	196.24	174.54	134.04	215.12
Sum min 11-60	8313.72	6851.75	5473.73	7857.58
Average min 11-60	166.27	137.04	109.47	157.15
Ambulatory Time
Sum min 1-60	608.60	504.20	422.40	632.20
Average min 1-60	10.14	8.40	7.04	10.54
Sum min 11-60	422.90	327.30	278.90	397.90
Average min 11-60	8.46	6.55	5.58	7.96
Ambulatory Counts
Sum min 1-60	4305	3797	2693	4863
Average min 1-60	71.75	63.28	44.88	81.05
Sum min 11-60	2945	2369	1737	2767
Average min 11-60	58.90	47.38	34.74	55.34
Ambulatory Episodes
Sum min 1-60	615	527	427	613
Average min 1-60	10.25	8.78	7.12	10.22
Sum min 11-60	449	364	305	412
Average min 11-60	8.98	7.28	6.10	8.24
Stereotypical Time
Sum min 1-60	622.40	536.70	558.50	562.70
Average min 1-60	10.37	8.95	9.31	9.38
Sum min 11-60	476.10	404.10	421.90	430.80
Average min 11-60	9.52	8.08	8.44	8.62
Stereotypical Counts
Sum min 1-60	3768	3297	3332	3533
Average min 1-60	62.80	54.95	55.53	58.88
Sum min 11-60	2835	2458	2506	2656
Average min 11-60	56.70	49.16	50.12	53.12
Rest Time
Sum min 1-60	2338.40	2497.70	2615	2354.20
Average min 1-60	38.97	41.63	43.58	39.24
Sum min 11-60	2015.10	2165.10	2235.80	2069.70
Average min 11-60	40.30	43.30	44.72	41.39
Vertical Counts
Sum min 1-60	224.50	220.40	121.60	412.10
Average min 1-60	3.74	3.67	2.03	6.87
Sum min 11-60	191.90	175.90	112.40	332.30
Average min 11-60	3.84	3.52	2.25	6.65
Vertical Time
Sum min 1-60	402	347	226	533
Average min 1-60	6.70	5.78	3.77	8.88
Sum min 11-60	319	259	194	373
Average min 11-60	6.38	5.18	3.88	7.46
Clockwise Rotation
Sum min 1-60	50	38	36	50
Average min 1-60	.83	.63	.60	.83
Sum min 11-60	38	26	22	30
Average min 11-60	.76	.52	.44	.60
Counter Clockwise Rotation
Sum min 1-60	57	59	38	74
Average min 1-60	.95	.98	.63	1.23
Sum min 11-60	35	41	28	40
Average min 11-60	.70	.82	.56	.80
Jump Counts
Sum min 1-60	30.10	61.30	4.10	50.90
Average min 1-60	.50	1.02	.07	.85
Sum min 11-60	25.40	43.50	3.40	41.60
Average min 11-60	.51	.87	.07	.83
Jump Time
Sum min 1-60	114	185	27	149
Average min 1-60	1.90	3.08	.45	2.48
Sum min 11-60	86	134	21	106
Average min 11-60	1.72	2.68	.42	2.12
Average Velocity
Sum min 1-60	NA	NA	NA	NA
Average min 1-60	21.47	23.97	20.32	23.04
Sum min 11-60
Average min 11-60	21.62	23.95	20.53	21.81

Zone Analysis

	CF1 control female 1	CF1 control female 2	CF1 control male 1	CF1 control male 2
Zone 0 Session Time
Sum min 1-60	2403.65	2467.55	2725.65	2140.95
Average min 1-60	40.06	41.13	45.43	35.68
Sum min 11-60	1946.80	2022	2203.60	1667.45
Average min 11-60	38.94	40.44	44.07	33.35
Zone 0 Entries
Sum min 1-60	1193	1350	939	843
Average min 1-60	19.88	22.50	15.65	14.05
Sum min 11-60	934	1027	738	628
Average min 11-60	18.68	20.54	14.76	12.56
Zone 1 Session Time
Sum min 1-60	1196.35	1132.45	874.35	1459.05
Average min 1-60	19.94	18.87	14.57	24.32
Sum min 11-60	993.20	918	736.40	1272.55
Average min 11-60	19.86	18.36	14.73	25.45
Zone 1 Entries
Sum min 1-60	1193	1350	939	843
Average min 1-60	19.88	22.50	15.65	14.05
Sum min 11-60	933	1027	737	627
Average min 11-60	18.66	20.54	14.74	12.54
	Mutant male 1	Mutant male 2	Mutant male 3	Mutant male 4
Zone 0 Session Time
Sum min 1-60	2729.60	2817.40	3131.60	2367.60
Average min 1-60	45.49	46.96	52.19	39.46
Sum min 11-60	2318.15	2271.75	2549.85	1877.95
Average min 11-60	46.36	45.44	51	37.56
Zone 0 Entries
Sum min 1-60	414	478	373	491
Average min 1-60	6.90	7.97	6.22	8.18
Sum min 11-60	252	342	260	352
Average min 11-60	5.04	6.84	5.20	7.04
Zone 1 Session Time
Sum min 1-60	2729.60	782.60	468.40	1232.40
Average min 1-60	45.49	13.04	7.81	20.54
Sum min 11-60	2318.15	668.25	390.15	1062.05
Average min 11-60	46.36	13.37	7.80	21.24
Zone 1 Entries
Sum min 1-60	414	478	373	490
Average min 1-60	6.90	7.97	6.22	8.17
Sum min 11-60	252	341	260	352
Average min 11-60	5.04	6.82	5.20	7.04
	Mutant female 1	Mutant female 2	Mutant female 3	Mutant female 4
Zone 0 Session Time
Sum min 1-60	2542.70	2973.10	2853.40	1982.80
Average min 1-60	42.38	49.60	47.56	33.05
Sum min 11-60	2012.80	2394.30	2313.40	1511.80
Average min 11-60	40.26	47.89	46.27	30.24
Zone 0 Entries
Sum min 1-60	584	319	426	604
Average min 1-60	9.73	5.32	7.10	10.07
Sum min 11-60	468	240	319	448
Average min 11-60	9.36	4.80	6.38	8.96
Zone 1 Session Time
Sum min 1-60	1057.30	623.90	746.60	1617.20
Average min 1-60	17.62	10.40	12.44	26.95
Sum min 11-60	927.20	545.70	626.60	1428.20
Average min 11-60	18.54	10.91	12.53	28.56
Zone 1 Entries
Sum min 1-60	585	319	426	604
Average min 1-60	9.75	5.32	7.10	10.07
Sum min 11-60	468	240	320	449
Average min 11-60	9.36	4.80	6.40	8.98
	129/sv control female 1	129/sv control female 2	129/sv control male 1	129/sv control male 2
Zone 0 Session Time
Sum min 1-60	2183.40	2672.20	2837.20	2292.20
Average min 1-60	36.39	44.54	47.29	38.20
Sum min 11-60	1821.60	2193.60	2285.90	1872.40
Average min 11-60	36.43	43.87	45.72	37.45
Zone 0 Entries
Sum min 1-60	522	400	254	408
Average min 1-60	8.70	6.67	4.23	6.80
Sum min 11-60	387	273	192	261
Average min 11-60	7.74	5.46	3.84	5.22
Zone 1 Session Time
Sum min 1-60	1416.60	927.80	762.80	1307.80
Average min 1-60	23.61	15.46	12.71	21.80
Sum min 11-60	1118.40	746.40	654.10	1067.60
Average min 11-60	22.37	14.93	13.08	21.35
Zone 1 Entries
Sum min 1-60	522	400	253	409
Average min 1-60	8.70	6.67	4.22	6.82
Sum min 11-60	388	272	191	261
Average min 11-60	7.76	5.44	3.82	5.22

Summary - Clinical Chemistry

No genotype associated abnormalties were observed in clinical chemistry analysis.

Complete Data - Clinical Chemistry

	CF1 control female 1	CF1 control female 2	CF1 control male 1	CF1 control male 2
Glucose (mg/dL)	143	170	240	206
Urea Nitrogen (mg/dL)	23	18	23	17
Creatinine (mg/dL)	0.2	0.3	0.3	0.2
Sodium (mmol/L)	156	156	154	150
Potassium (mmol/L)	6.3	7.7	7.6	9.4
Chloride (mmol/L)	109	110	109	108
Total Protein (g/dL)	5.8	5.6	5.4	5.5
Albumin (g/dL)	3.7	3.5	3.3	3.3
Globulin (g/dL)	2.1	2.1	2.1	2.2
Calcium (mg/dL)	10.8	10.8	10.7	10.2
Cholesterol (mg/dL)	103	105	128	122
Total Bili (mg/dL)	0.2	0.2	0.1	0.2
ALT (U/L)	42	79	35	38
ALP (U/L)	221	192	134	117
	Mutant male 1	Mutant male 2	Mutant male 3	Mutant male 4
Glucose (mg/dL)	156	121	151	172
Urea Nitrogen (mg/dL)	20	22	23	25
Creatinine (mg/dL)	0.2	0.1	0.1	0.1
Sodium (mmol/L)	157	153	154	150
Potassium (mmol/L)	7.0	QNS	9.4	10
Chloride (mmol/L)	105	107	106	108
Total Protein (g/dL)	5.8	6.1	6.3	6.2
Albumin (g/dL)	3.6	3.9	4.0	3.9
Globulin (g/dL)	2.2	2.2	2.3	2.3
Calcium (mg/dL)	11.3	9.7	10.2	10.1
Cholesterol (mg/dL)	129	101	140	134
Total Bili (mg/dL)	0.2	0.7	0.6	0.5
ALT (U/L)	28	40	46	40
ALP (U/L)	129	174	108	90
	Mutant female 1	Mutant female 2	Mutant female 3	Mutant female 4
Glucose (mg/dL)	QNS	QNS	QNS	QNS
Urea Nitrogen (mg/dL)	QNS	22	21	QNS
Creatinine (mg/dL)	QNS	QNS	QNS	QNS
Sodium (mmol/L)	161	QNS	QNS	QNS
Potassium (mmol/L)	5.8	QNS	QNS	QNS
Chloride (mmol/L)	109	QNS	QNS	QNS
Total Protein (g/dL)	QNS	QNS	QNS	QNS
Albumin (g/dL)	QNS	3.8	3.6	3.7
Globulin (g/dL)	QNS	QNS	QNS	QNS
Calcium (mg/dL)	QNS	11.1	10.8	QNS
Cholesterol (mg/dL)	QNS	QNS	86	QNS
Total Bili (mg/dL)	QNS	0.2	0.2	0.2
ALT (U/L)	QNS	153	63	33
ALP (U/L)	QNS	189	200	205
	129/sv control female 1	129/sv control female 2	129/sv control male 1	129/sv control male 2
Glucose (mg/dL)	QNS	QNS	QNS	QNS
Urea Nitrogen (mg/dL)	27	26	27	24
Creatinine (mg/dL)	QNS	QNS	QNS	QNS
Sodium (mmol/L)	161	150	147	152
Potassium (mmol/L)	8.8	7.5	8.5	8.9
Chloride (mmol/L)	117	109	105	107
Total Protein (g/dL)	QNS	QNS	QNS	QNS
Albumin (g/dL)	QNS	QNS	QNS	QNS
Globulin (g/dL)	QNS	QNS	QNS	QNS
Calcium (mg/dL)	QNS	QNS	QNS	QNS
Cholesterol (mg/dL)	QNS	QNS	QNS	QNS
Total Bili (mg/dL)	QNS	QNS	QNS	QNS
ALT (U/L)	33	24	37	28
ALP (U/L)	QNS	QNS	QNS	QNS

Comments:  QNS=Quantity not sufficient to perform all tests Samples 1-4 and 9-13 had slight hemolysis. Samples 6-8 had gross hemolysis which may affect the results.

Summary - Hematology

No genotype associated hematologic abnormalties were observed.

Complete Data - Hematology

	CF1 control female 1	CF1 control female 2	CF1 control male 1	CF1 control male 2
Leukocytes
WBC (x10³/μL)	3.00	7.18	5.06	6.74
Manual differential data
Segmented Neutrophils (%)	13.00	16.00	25.00	15.00
Band Neutrophils (%)	0.00	0.00	0.00	0.00
Lymphocytes (%)	83.00	83.00	69.00	81.00
Monocytes (%)	3.00	1.00	4.00	2.00
Eosinophils (%)	1.00	0.00	2.00	2.00
Basophils (%)	0.00	0.00	0.00	0.00
Nucleated RBCx (/100RBC)	0.00	0.00	0.00	0.00
Absolute leukocytes numbers calculated from manual differential
Segmented Neutrophils (x10³/μL)	0.39	1.15	1.27	1.01
Band Neutrophils (x10³/μL)	0.00	0.00	0.00	0.00
Lymphocytes (x10³/μL)	2.49	5.96	3.49	5.46
Monocytes (x10³/μL)	0.09	0.07	0.20	0.13
Eosinophils (x10³/μL)	0.03	0.00	0.10	0.13
Basophils (x10³/μL)	0.00	0.00	0.00	0.00
Differential data generated by HemaVet
Segmented Neutrophils (%)	14.73	30.67	13.95	10.77
Lymphocytes (%)	80.53	62.00	76.43	79.86
Monocytes (%)	4.56	6.42	9.31	8.78
Eosinophils (%)	0.14	0.71	0.24	0.56
Basophils (%)	0.05	0.19	0.07	0.03
Absolute leukocytes numbers calculated from HemaVet differential
Segmented Neutrophils (x10³/μL)	0.44	2.20	0.71	0.73
Lymphocytes (x10³/μL)	2.42	4.45	3.87	5.38
Monocytes (x10³/μL)	0.14	0.46	0.47	0.59
Eosinophils (x10³/μL)	0.00	0.05	0.01	0.04
Basophils (x10³/μL)	0.00	0.01	0.00	0.00
Red blood cell parameters
RBC (M/μL)	8.30	10.04	8.09	8.85
Hemoglobin (g/dL)	13.40	15.60	13.10	14.50
Hematocrit (%)	49.50	56.20	46.80	50.70
MCV (fL)	59.60	56.00	57.80	57.30
MCH (pg)	16.10	15.50	16.20	16.40
MCHC (g/dL)	27.10	27.80	28.00	28.60
Red Cell Distribution Width (%)	19.30	19.00	19.10	18.40
Platelet parameters
Platelets (x10³/μL)	657.00	589.00	771.00	797.00
MPV (fL)	4.90	5.40	4.90	5.10
	Mutant male 1	Mutant male 2	Mutant male 3	Mutant male 4
Leukocytes
WBC (x10³/μL)	4.38	5.46	4.22	4.84
Manual differential data
Segmented Neutrophils (%)	28.00	10.00	13.00	19.00
Band Neutrophils (%)	0.00	0.00	0.00	0.00
Lymphocytes (%)	59.00	84.00	82.00	72.00
Monocytes (%)	12.00	5.00	5.00	8.00
Eosinophils (%)	1.00	1.00	0.00	1.00
Basophils (%)	0.00	0.00	0.00	0.00
Nucleated RBCx (/100RBC)	0.00	0.00	0.00	0.00
Absolute leukocytes numbers calculated from manual differential
Segmented Neutrophils (x10³/μL)	1.23	0.55	0.55	0.92
Band Neutrophils (x10³/μL)	0.00	0.00	0.00	0.00
Lymphocytes (x10³/μL)	2.58	4.59	3.46	3.48
Monocytes (x10³/μL)	0.53	0.27	0.21	0.39
Eosinophils (x10³/μL)	0.04	0.05	0.00	0.05
Basophils (x10³/μL)	0.00	0.00	0.00	0.00
Differential data generated by HemaVet
Segmented Neutrophils (%)	11.33	13.26	17.35	13.58
Lymphocytes (%)	80.14	74.75	74.36	78.10
Monocytes (%)	6.44	11.40	7.04	7.25
Eosinophils (%)	1.43	0.59	0.86	1.04
Basophils (%)	0.67	0.00	0.39	0.04
Absolute leukocytes numbers calculated from HemaVet differential
Segmented Neutrophils (x10³/μL)	0.50	0.72	0.73	0.66
Lymphocytes (x10³/μL)	3.51	4.08	3.14	3.78
Monocytes (x10³/μL)	0.28	0.62	0.30	0.35
Eosinophils (x10³/μL)	0.06	0.03	0.04	0.05
Basophils (x10³/μL)	0.03	0.00	0.02	0.00
Red blood cell parameters
RBC (M/μL)	9.26	7.88	8.72	9.11
Hemoglobin (g/dL)	17.00	14.80	15.90	16.40
Hematocrit (%)	60.30	54.00	57.40	60.70
MCV (fL)	65.10	68.50	65.80	66.60
MCH (pg)	18.40	18.80	18.20	18.00
MCHC (g/dL)	28.20	27.40	27.76	27.00
Red Cell Distribution Width (%)	16.40	18.10	17.40	17.30
Platelet parameters
Platelets (x10³/μL)	546.00	355.00	550.00	480.00
MPV (fL)	4.10	4.10	4.00	4.00
	Mutant female 1	Mutant female 2	Mutant female 3	Mutant female 4
Leukocytes
WBC (x10³/μL)	5.70	5.98	4.12	6.82
Manual differential data
Segmented Neutrophils (%)	22.00	37.00	17.00	19.00
Band Neutrophils (%)	0.00	0.00	0.00	0.00
Lymphocytes (%)	76.00	60.00	80.00	77.00
Monocytes (%)	2.00	3.00	3.00	2.00
Eosinophils (%)	0.00	0.00	0.00	2.00
Basophils (%)	0.00	0.00	0.00	0.00
Nucleated RBCx (/100RBC)	0.00	0.00	0.00	0.00
Absolute leukocytes numbers calculated from manual differential
Segmented Neutrophils (x10³/μL)	1.25	2.21	0.70	1.30
Band Neutrophils (x10³/μL)	0.00	0.00	0.00	0.00
Lymphocytes (x10³/μL)	4.33	3.59	3.30	5.25
Monocytes (x10³/μL)	0.11	0.18	0.12	0.14
Eosinophils (x10³/μL)	0.00	0.00	0.00	0.14
Basophils (x10³/μL)	0.00	0.00	0.00	0.00
Differential data generated by HemaVet
Segmented Neutrophils (%)	12.34	17.71	9.58	13.79
Lymphocytes (%)	77.24	70.19	79.02	75.77
Monocytes (%)	8.11	11.23	10.54	8.46
Eosinophils (%)	1.72	0.81	0.68	1.51
Basophils (%)	0.58	0.06	0.18	0.46
Absolute leukocytes numbers calculated from HemaVet differential
Segmented Neutrophils (x10³/μL)	0.70	1.06	0.39	0.94
Lymphocytes (x10³/μL)	4.40	4.20	3.26	5.17
Monocytes (x10³/μL)	0.46	0.67	0.43	0.58
Eosinophils (x10³/μL)	0.10	0.05	0.03	0.10
Basophils (x10³/μL)	0.03	0.00	0.01	0.03
Red blood cell parameters
RBC (M/μL)	8.94	9.38	8.31	9.44
Hemoglobin (g/dL)	16.70	18.10	15.90	18.30
Hematocrit (%)	58.40	62.70	56.30	63.80
MCV (fL)	65.30	66.80	67.70	67.60
MCH (pg)	18.70	19.30	19.10	19.40
MCHC (g/dL)	28.60	28.90	28.20	28.70
Red Cell Distribution Width (%)	17.60	17.90	18.00	17.70
Platelet parameters
Platelets (x10³/μL)	609.00	508.00	474.00	565.00
MPV (fL)	4.50	4.20	4.30	4.30

	129/sv control female 1	129/sv control female 2	129/sv control male 1	129/sv control male 2
Leukocytes
WBC (x10³/μL)	6.64	8.16	7.36	8.02
Manual differential data
Segmented Neutrophils (%)	5.00	4.00	6.00	8.00
Band Neutrophils (%)	0.00	0.00	0.00	0.00
Lymphocytes (%)	89.00	93.00	91.00	85.00
Monocytes (%)	5.00	3.00	2.00	6.00
Eosinophils (%)	1.00	0.00	1.00	1.00
Basophils (%)	0.00	0.00	0.00	0.00
Nucleated RBCx (/100RBC)	0.00	0.00	0.00	0.00
Absolute leukocytes numbers calculated from manual differential
Segmented Neutrophils (x10³/μL)	0.33	0.33	0.44	0.64
Band Neutrophils (x10³/μL)	0.00	0.00	0.00	0.00
Lymphocytes (x10³/μL)	5.91	7.59	6.70	6.82
Monocytes (x10³/μL)	0.33	0.24	0.15	0.48
Eosinophils (x10³/μL)	0.07	0.00	0.07	0.08
Basophils (x10³/μL)	0.00	0.00	0.00	0.00
Differential data generated by HemaVet
Segmented Neutrophils (%)	11.86	16.86	9.51	9.59
Lymphocytes (%)	81.21	74.75	85.20	85.82
Monocytes (%)	6.12	5.75	4.27	4.10
Eosinophils (%)	0.58	1.98	0.88	0.43
Basophils (%)	0.23	0.66	0.15	0.05
Absolute leukocytes numbers calculated from HemaVet differential
Segmented Neutrophils (x10³/μL)	0.79	1.38	0.70	0.77
Lymphocytes (x10³/μL)	5.39	6.10	6.27	6.88
Monocytes (x10³/μL)	0.41	0.47	0.31	0.33
Eosinophils (x10³/μL)	0.04	0.16	0.06	0.03
Basophils (x10³/μL)	0.02	0.05	0.01	0.00
Red blood cell parameters
RBC (M/μL)	9.53	9.27	9.16	9.23
Hemoglobin (g/dL)	16.30	16.50	15.80	16.30
Hematocrit (%)	59.60	62.40	60.90	60.70
MCV (fL)	62.50	67.30	66.50	65.80
MCH (pg)	17.10	17.80	17.20	17.70
MCHC (g/dL)	27.30	26.40	25.90	26.90
Red Cell Distribution Width (%)	18.40	17.90	17.70	18.50
Platelet parameters
Platelets (x10³/μL)	36.30	379.00	402.00	430.00
MPV (fL)	4.60	4.80	4.10	4.20