Summary of Phenotype

Results of this report are primarily descriptive and represent the analysis of one mutant mouse. They should therefore be interpreted with caution and as preliminary, observation-generating data.

Anecdotally, BMBX mutant mice have been reported to have low testicular weights and absence of mature sperm. In this study, the left testicle of the mutant was grossly small and was less than half the weight of the control animal. On histopathologic examination, diffuse germ cell atrophy with absence of spermatogenesis and interstitial cell hyperplasia was found. No other significant phenotype abnormalities were identified.

Summary - Gross Findings

The mutants left testicular weight (0.0322g) was less than half of the weight of the left testicle of the control animal (0.1081g).

Phenotypic Abnormalities - Gross Findings
BMBX (unofficial nomenclature)

Finding	Sex	Age	# Affected	Image(s)
testicular atrophy	M	16w	1/1

Click on image for larger view.

Organ/Body Weights

	BMBX	C57BL/6J control
adrenal gland		0.0024
adrenal glands	0.0036
body weight	30.56	29.3
brain	0.5020
heart	0.2178	0.1792
left kidney	0.2087	0.2538
left testicle (without epididymis)	0.0322	0.1081
liver	1.8238	1.1637
spleen	0.0850	0.0791

Summary - Histology

The testes had diffuse germ cell atrophy with absence of spermatogenesis and interstitial cell hyperplasia.

Phenotypic Abnormalities - Histology
BMBX (unofficial nomenclature)

Organ	Lesion	Sex	Age	# Affected	Image(s)
testes	marked diffuse interstitial cell hyperplasia	M	16w	1/1
testes	germ cell atrophy with absence of spermatogenesis	M	16w	1/1

Click on lesion for full description. Click on image for larger view.

Complete Data - Histology

(Click on lesion for full description.)

	BMBX	C57BL/6J control
adrenal gland(s)
no lesions	-	-
aorta
no lesions	-	-
brown fat
no lesions	-	-
bulbourethral gland
no lesions	-	-
cecum
no lesions	-	-
cerebellum
no lesions	-	-
cerebrum
no lesions	-	-
coagulating gland
no lesions	-	-
costrochondral junction
no lesions	-	-
diaphragm
no lesions	-	-
distal colon
no lesions	-	-
duodenum
no lesions	-	-
epididymis
no lesions	-	-
esophagus
no lesions	-
exorbital gland
no lesions	-	-
eye
nuclear cataract	-	+
femoral bone marrow
no lesions	-	-
femur
no lesions	-	-
gallbladder
no lesions	-
Harderian gland
no lesions	-	-
heart
no lesions	-	-
ileum
no lesions	-	-
incisor tooth
no lesions	-	-
inner ear
no lesions	-	-
jejunum
no lesions	-	-
kidneys
peripelvic lymphoid infiltrate	+	-
lacrimal gland(s)
no lesions	-	-
liver
mild focal granulopoiesis	+	-
lung
peribronchiolar lymphoid infiltrate	+	-
subpleural lymphoid aggregate	+	-
mammary gland
no lesions	-	-
mesenteric lymph node
no lesions	-	-
molar tooth
no lesions	-	-
nasal cavity
no lesions	-	-
pancreas
no lesions	-	-
parathyroid gland
no lesions	-	-
parotid salivary glands
no lesions	-	-
pituitary gland
no lesions	-	-
preputial glands
no lesions	-	-
prostate gland
no lesions	-	-
proximal colon
no lesions	-	-
quadriceps muscle
no lesions	-	-
sciatic nerve
no lesions	-	-
skin
no lesions	-	-
spinal cord
no lesions	-	-
spleen
no lesions	-	-
stifle
no lesions	-	-
stomach
no lesions	-	-
sublingual salivary gland
no lesions	-	-
submand. salivary glands
no lesions	-	-
testes
germ cell atrophy with absence of spermatogenesis	+	-
marked diffuse interstitial cell hyperplasia	+	-
thymus
no lesions	-	-
thyroid gland
no lesions	-	-
tongue
no lesions	-	-
trachea
no lesions	-	-
urethra
no lesions	-	-
urinary bladder
no lesions	-	-
vesicular gland
no lesions	-	-

Summary - Behavioral Testing

A series of behavioral tests were performed on the mutant and control mouse. Both mice responded within normal limits and no differences were observed between the two.

Complete Data - Behavioral Testing

Reflex Testing

	BMBX	C57BL/6J control
visible	wnl
spontaneous behavior	wnl	wnl
reaching reflex	wnl	wnl
righting reflex	wnl
trunk curl	wnl	wnl
negative geotaxis	wnl	wnl
hanging wire grip test	wnl
preyer reflex	wnl	wnl
pinna reflex	wnl	wnl
vibrissae reflex	wnl	wnl

 

Summary - Clinical Chemistry

No genotype associated abnormalties were observed in clinical chemistry analysis

Complete Data - Clinical Chemistry

	BMBX	C57BL/6J control
Glucose (mg/dL)	238	261
Urea Nitrogen (mg/dL)	34	15
Creatinine (mg/dL)	0.2	0.2
Sodium (mmol/L)	144	144
Potassium (mmol/L)	4.1	4.3
Chloride (mmol/L)	110	112
Total Protein (g/dL)	4.7	4.0
Albumin (g/dL)	2.2	1.7
Globulin (g/dL)	2.6	2.3
Calcium (mg/dL)	9.3	8.9
Phosphorus (mg/dL)		9.5
Cholesterol (mg/dL)	94	45
Total Bili (mg/dL)		0.5
ALT (U/L)	47	69
ALP (U/L)	86	86
Total CO2 (mmol/L)		19
Anion Gap (mmol/L)		17

Summary - Hematology

No genotype associated hematologic abnormalties were observed.

Complete Data - Hematology

	BMBX	C57BL/6J control
Leukocytes
WBC (x10³/μL)	1.94	4.88
Manual differential data
Segmented Neutrophils (%)	6.00	6.00
Band Neutrophils (%)	0.00	0.00
Lymphocytes (%)	91.00	88.00
Monocytes (%)	2.00	6.00
Eosinophils (%)	1.00	0.00
Basophils (%)	0.00	0.00
Nucleated RBCx (/100RBC)	0.00	0.00
Absolute leukocytes numbers calculated from manual differential
Segmented Neutrophils (x10³/μL)	0.12	0.29
Band Neutrophils (x10³/μL)	0.00	0.00
Lymphocytes (x10³/μL)	1.77	4.29
Monocytes (x10³/μL)	0.04	0.29
Eosinophils (x10³/μL)	0.02	0.00
Basophils (x10³/μL)	0.00	0.00
Differential data generated by HemaVet
Segmented Neutrophils (%)	13.52	38.89
Lymphocytes (%)	82.13	57.99
Monocytes (%)	3.72	2.44
Eosinophils (%)	0.53	0.50
Basophils (%)	0.10	0.19
Absolute leukocytes numbers calculated from HemaVet differential
Segmented Neutrophils (x10³/μL)	0.26	1.90
Lymphocytes (x10³/μL)	1.59	2.83
Monocytes (x10³/μL)	0.07	0.12
Eosinophils (x10³/μL)	0.01	0.02
Basophils (x10³/μL)	0.00	0.01
Red blood cell parameters
RBC (M/μL)	8.79	10.84
Hemoglobin (g/dL)	12.20	13.50
Hematocrit (%)	46.90	56.00
MCV (fL)	53.30	51.70
MCH (pg)	13.90	12.50
MCHC (g/dL)	26.00	24.10
Red Cell Distribution Width (%)	16.90	21.10
Platelet parameters
Platelets (x10³/μL)	166.00	625.00
MPV (fL)	5.20	4.70

Complete Urinalysis Data

	BMBX	C57BL/6J control
collection	voided
clarity	clear	WNL
color	pale yello	WNL
glucose	QNS	-
pH	6.5	6.0
specific gravity	1.083	1.068
protein	QNS	++
blood	QNS	-
sediment exam	QNS	QNS
bilirubins	QNS	-
ketones	QNS	QNS
urobilirubins	QNS	QNS

(LEGEND:   + = positive    - = negative    ~ = suspect    QNS = quantity not sufficient    WNL = within normal limits)