Summary of Phenotype

Results of this report are primarily descriptive and represent the analysis of one mutant mouse. They should therefore be interpreted with caution and as preliminary, observation-generating data.

Anecdotally, BMB17 mutant mice been reported to have sperm heads with no tails. In this study, diffuse aspermia and scattered germinal testicular epithelial degeneration, moderate preputial gland acinar atrophy with diffuse ductal ectasia, and bulbourethral gland metaplasia/atrophy with abnormal mucin production were identified. No other significant phenotype abnormalities were identified.

Summary - Gross Findings

No gross lesions were observed in the control or mutant.

Organ/Body Weights

	BMB17	C57BL/6J control
adrenal glands	.0065g
adrenal glands (single)		0.0024g
body weight	25.97g	29.3g
brain	.4584g
heart	.1600g	0.1792g
left kidney	.1691g	0.2538g
left testicle	.0750g	0.1018g
liver	1.3446g	1.1637g
spleen	.0650g	0.0791g

Summary - Histology

The mutant had diffuse aspermia (no mature sperm in the tubular lumens) in the testes and epididymis. While most seminiferous tubules had normal appearing germ cells including spermatogonia, primary and secondary spermatocytes, and spermatids, a few scattered tubules had mild to marked germinal epithelial degeneration.
There was moderate acinar atrophy with diffuse ductal ectasia in the preputial glands. The bulbourethral gland had acinar epithelial metaplasia/atrophy and did not stain with Alcian Blue and only half of the acini stained positively for PAS. In contrast, the bulbourethral gland of the control animal stained Alcian Blue positive (acidic mucins) and PAS positive (neutral mucins). This suggests that the bulbourethral gland of the mutant had decreased and altered mucin product.

Phenotypic Abnormalities - Histology
BMB17 (unofficial nomenclature)

Organ	Lesion	Sex	Age	# Affected	Image(s)
preputial glands	acinar atrophy with diffuse ductal ectasia	M	18w	1/1
epididymis	diffuse aspermia	M	18w	1/1
testes	diffuse aspermia	M	18w	1/1
testes	scattered germinal epithelial degeneration	M	18w	1/1
bulbourethral gland	bulbourethral gland metaplasia and atrophy	M	18w	1/1

Click on lesion for full description. Click on image for larger view.

Complete Data - Histology

(Click on lesion for full description.)

	BMB17	C57BL/6J control
adrenal gland(s)
no lesions	-	-
aorta
no lesions	-	-
brown fat
no lesions	-	-
bulbourethral gland
bulbourethral gland metaplasia and atrophy	+	-
cecum
no lesions	-	-
cerebellum
no lesions	-	-
cerebrum
no lesions	-	-
coagulating gland
no lesions	-	-
costrochondral junction
no lesions	-	-
diaphragm
no lesions	-	-
distal colon
no lesions	-	-
duodenum
no lesions	-	-
epididymis
diffuse aspermia	+	-
esophagus
no lesions	-
exorbital gland
no lesions	-	-
eye
nuclear cataract	-	+
femoral bone marrow
no lesions	-	-
femur
no lesions	-	-
gallbladder
no lesions	-
Harderian gland
no lesions	-	-
heart
no lesions	-	-
ileum
no lesions	-	-
incisor tooth
no lesions	-	-
inner ear
no lesions	-	-
jejunum
no lesions	-	-
kidneys
no lesions	-	-
lacrimal gland(s)
no lesions	-	-
liver
no lesions	-	-
lung
no lesions	-	-
mammary gland
no lesions	-	-
mandibular lymph node(s)
no lesions	-
mesenteric lymph node
no lesions	-
molar tooth
no lesions	-	-
nasal cavity
no lesions	-	-
pancreas
no lesions	-	-
parathyroid gland
no lesions	-	-
parotid salivary glands
no lesions	-	-
pituitary gland
no lesions	-	-
preputial glands
acinar atrophy with diffuse ductal ectasia	+	-
prostate gland
no lesions	-	-
proximal colon
no lesions	-	-
quadriceps muscle
no lesions	-	-
sciatic nerve
no lesions	-	-
skin
no lesions	-	-
spinal cord
no lesions	-	-
spleen
no lesions	-	-
stifle
no lesions	-	-
stomach
no lesions	-	-
sublingual salivary gland
no lesions	-	-
submand. salivary glands
no lesions	-	-
testes
diffuse aspermia	+	-
scattered germinal epithelial degeneration	+	-
thymus
no lesions	-	-
thyroid gland
no lesions	-	-
tongue
no lesions	-	-
trachea
no lesions	-	-
urethra
no lesions	-	-
urinary bladder
no lesions	-	-
vesicular gland
no lesions	-	-

Summary - Behavioral Testing

A series of behavioral tests were performed on the mutant and control mouse. Both mice responded within normal limits and no differences were observed between the two.

Complete Data - Behavioral Testing

Reflex Testing

	BMB17	C57BL/6J control
visible	wnl
spontaneous behavior	wnl	wnl
reaching reflex	wnl	wnl
righting reflex	wnl
trunk curl	wnl	wnl
negative geotaxis	wnl	wnl
hanging wire grip test	wnl
preyer reflex	wnl	wnl
pinna reflex	wnl	wnl
vibrissae reflex	wnl	wnl
pupillary light reflex	wnl

 

Summary - Clinical Chemistry

No genotype associated abnormalties were observed in clinical chemistry analysis.

Complete Data - Clinical Chemistry

	BMB17	C57BL/6J control
Glucose (mg/dL)	255	261
Urea Nitrogen (mg/dL)	26	15
Creatinine (mg/dL)	0.2	0.2
Sodium (mmol/L)	146	144
Potassium (mmol/L)	4.0	4.3
Chloride (mmol/L)	111	112
Total Protein (g/dL)	4.8	4.0
Albumin (g/dL)	2.2	1.7
Globulin (g/dL)	2.6	2.3
Calcium (mg/dL)	9.1	8.9
Phosphorus (mg/dL)		9.5
Cholesterol (mg/dL)	79	45
Total Bili (mg/dL)		0.5
ALT (U/L)	50	69
ALP (U/L)	105	86
Total CO2 (mmol/L)		19
Anion Gap (mmol/L)		17

Summary - Hematology

No genotype associated hematologic abnormalties were observed.

Complete Data - Hematology

	BMB17	C57BL/6J control
Leukocytes
WBC (x10³/μL)	6.42	4.88
Manual differential data
Segmented Neutrophils (%)	3.00	6.00
Band Neutrophils (%)	0.00	0.00
Lymphocytes (%)	95.00	88.00
Monocytes (%)	2.00	6.00
Eosinophils (%)	0.00	0.00
Basophils (%)	0.00	0.00
Nucleated RBCx (/100RBC)	0.00	0.00
Absolute leukocytes numbers calculated from manual differential
Segmented Neutrophils (x10³/μL)	0.19	0.29
Band Neutrophils (x10³/μL)	0.00	0.00
Lymphocytes (x10³/μL)	6.10	4.29
Monocytes (x10³/μL)	0.13	0.29
Eosinophils (x10³/μL)	0.00	0.00
Basophils (x10³/μL)	0.00	0.00
Differential data generated by HemaVet
Segmented Neutrophils (%)	7.52	38.89
Lymphocytes (%)	89.23	57.99
Monocytes (%)	3.04	2.44
Eosinophils (%)	0.12	0.50
Basophils (%)	0.09	0.19
Absolute leukocytes numbers calculated from HemaVet differential
Segmented Neutrophils (x10³/μL)	0.48	1.90
Lymphocytes (x10³/μL)	5.73	2.83
Monocytes (x10³/μL)	0.20	0.12
Eosinophils (x10³/μL)	0.01	0.02
Basophils (x10³/μL)	0.01	0.01
Red blood cell parameters
RBC (M/μL)	8.73	10.84
Hemoglobin (g/dL)	12.60	13.50
Hematocrit (%)	46.50	56.00
MCV (fL)	53.30	51.70
MCH (pg)	14.40	12.50
MCHC (g/dL)	27.10	24.10
Red Cell Distribution Width (%)	17.90	21.10
Platelet parameters
Platelets (x10³/μL)	585.00	625.00
MPV (fL)	4.90	4.70

Complete Urinalysis Data

	BMB17	C57BL/6J control
collection	voided
clarity	clear	WNL
color	pale yellow	WNL
glucose	-	-
pH	6.5	6.0
specific gravity	1.065	1.068
protein	+	++
blood	-	-
sediment exam	WNL	QNS
bilirubins	-	-
ketones	-	QNS
urobilirubins	0.2	QNS

(LEGEND:   + = positive    - = negative    ~ = suspect    QNS = quantity not sufficient    WNL = within normal limits)