Username: 
Password: 
 

• About on-line access  • Apply for UserID

RADIL Introduces Pinworm by PCR Testing
11.19.2009

In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam.  With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results.  RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation.  In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).

Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay.  For more information and pricing, please click here. 

( for more info click here )
MFI2 - The next generation in serology testing
11.19.2009

At this year's National AALAS Meeting in Denver, Colorado, RADIL introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents.  MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available.   By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results.  Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.

For more information regarding MFI2, please visit the Serology section of this site.

( for more info click here )
Suggested General Breeding Strategy (B6 & FVB)
Donor: B6.mut-/- Recipient: FVB
Generate F1

Timeline

4 wks to get pups
7-8 wks for pups to reach sexual maturity
= ~ 3 months

2 cages: 1 FVB female + 1 B6.mut-/-male
8-12 pups/litter X 2 = 16-24 F1 pups

  • Cull males
  • Confirm mutant genotype (=heterozygous) of females
  • Use females for next round of breeding
Generate N2

Timeline

4 wks to get pups
7-8 wks for pups to reach sexual maturity
= ~ 3 months
Note: Y is now “fixed”
= YFVB

4 cages: 2 F1 FVB.B6.mut+/-females + 1 FVB male
8-12 pups/litter X 8 = 64-96 N2 pups

  • Cull females
  • Tail snip, make DNA, genotype males. Cull mut+/+
  • Genome scan analysis on mut+/-males (10 max)
  • Use “best” (= highest percent recipient genome) males for next round of breeding
Generate N3

Timeline

4 wks to get pups
7-8 wks for pups to reach sexual maturity
= ~ 3 months
Note: X is now “fixed”
= XFVB

4 cages: 2 FVB females + 1 N2 FVB.B6.mut+/-male
8-12 pups/litter X 8 = 64-96 N3 pups

  • Cull females
  • Tail snip, make DNA, genotype males. Cull mut+/+
  • Genome scan analysis on mut+/-males (10 max)
  • Use “best” (= highest percent recipient genome) males for next round of breeding

Continue this same process for 2 more generations (N4, N5). You can decrease your breeding cages to 2 and perform genome analysis on 5 mut+/-males at this point. By N5, the animals should be > 99% FVB. At this point, N5 animals can be intercrossed to generate homozygous mutants. It is also recommended that the colony continue to be backcrossed indefinitely to FVB to help minimize the residual donor DNA that flanks the mutation and gets carried along because it is closely linked to the mutant allele.
University of Missouri-Columbia
©2007 - Curators of the University of Missouri. All rights reserved. DMCA and other copyright information.
An equal opportunity/affirmative action institution.
design provided by Nine Plus One