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RADIL Introduces Pinworm by PCR Testing
11.19.2009

In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam.  With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results.  RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation.  In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).

Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay.  For more information and pricing, please click here. 

( for more info click here )
MFI2 - The next generation in serology testing
11.19.2009

At this year's National AALAS Meeting in Denver, Colorado, RADIL introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents.  MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available.   By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results.  Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.

For more information regarding MFI2, please visit the Serology section of this site.

( for more info click here )
Procedure for Making a Blood Smear

Peripheral blood or potassium EDTA anticoagulated blood (1-2 mg EDTA/1 ml blood) may be used. Smears of peripheral blood must be made immediately. Smears made from EDTA-anticoagulated blood should be made within 2 hours of collection. All specimens must be free of clots.

 
Step 1. Place a 2-3mm drop of blood about 1cm from the frosted end of a clean slide that is on a flat surface.

If using a needle and syringe, first remove the needle and then touch the end of the syringe to the slide.
   
If using a capillary tube, be sure to fill the capillary at least two-thirds with blood. Touch the end of the capillary to the slide.
   
Step 2. With the thumb and forefinger of the right hand, hold the end of a second slide (“spreader”) against the surface of the first slide at an angle of 30-45 degrees.
   
Step 3. Draw it back to contact the drop of blood. Allow the blood to spread and fill the angle between the two slides.
   
Step 4. Push the “spreader” slide at a moderate speed forward until all of the blood has been spread into a moderately thin film.
 
 
Alternatively, the slide may rest on the forefinger of the left hand. This may make it easier to maintain a moderate and consistent speed when “pushing” the “spreader” slide.
   
Note: The film should not cover the entire surface of the slide. In a good film, there is a thick portion and a thin portion and a gradual transition from one to the other. The film should have a smooth, even appearance and be free from ridges, waves, or holes. The end of the smear (the “feathered edge”) should be smooth and even. The edge of the “spreader” must be absolutely smooth. If it is rough, the film has ragged tails containing many leukocytes.
   
Shipping containers are available from most laboratory supply companies. It is important that the containers are sealed securely so that the slides won’t fall out during shipping.


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