RADIL - Research Animal Diagnostic Laboratory - Standard Operating Procedures

Teaching & Training Mission Teaching Resources Comparative Medicine Training

Survival Blood Collection SOP Procedure for Making a Blood Smear Proper technique for inflation of rodent lungs for histopathologic analysis Collection of rat mesenteric lymph nodes Collection of rodent gastrointestinal tracts Collection of Guinea pig submandibular salivary glands sdfgs df gdf g

RADIL Introduces Pinworm by PCR Testing
11.19.2009

In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam. With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results. RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation. In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).

Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay. For more information and pricing, please click here.

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MFI² - The next generation in serology testing
11.19.2009

At this year's National AALAS Meeting in Denver, Colorado, RADIL introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents. MFI² represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available. By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results. Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.

For more information regarding MFI², please visit the Serology section of this site.

( for more info click here )

Collection of rodent gastrointestinal tracts

	Step 1. Expose the peritoneal cavity by reflecting the skin and subcutaneous tissue.
	Step 2. Cut the stomach at the border of the nonglandular and glandular portion.
	Step 3. Grasp the stomach and cut the duodenum a few centimeters from the pylorus.
	Step 4. Remove the jejunum by trimming away the mesentery until the entire length is exposed.
	Step 5. Cut the ileum several centimeters from the cecum to free the jejunum.
	Step 6. Cut the anterior colon a few centimeters from the cecum and remove the ileum/cecum/colon complex.
	Step 7. Remove a section of the distal colon.
	Step 8. Fill a syringe with formalin and place the needle into the lumen of the stomach. Flush out all contents from the stomach and duodenum and place the stomach/duodenum in formalin.
	Step 9. Place the needle tip in the lumen of the jejunum and grasp the needle with forceps (to prevent the backflow of formalin). Flush all contents out of the jejunum and place the gut rull in formalin.
	Step 10. Place the tip of the needle in the lumen of the ileum, colon, or through the wall of the cecum and flush all contents out. Place the ileum/cecum/colon in formalin.
	Step 11. Place the tip of the needle in the distal colon and flush out any fecal pellets. Place the distal colon in formalin.
	Step 12. Here is an example of what the 4 tissues look like after flushing (stomach at top, jejunum in middle, and cecum and colon at the end).

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