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RADIL Introduces Pinworm by PCR Testing
11.19.2009

In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam.  With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results.  RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation.  In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).

Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay.  For more information and pricing, please click here. 

( for more info click here )
MFI2 - The next generation in serology testing
11.19.2009

At this year's National AALAS Meeting in Denver, Colorado, RADIL introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents.  MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available.   By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results.  Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.

For more information regarding MFI2, please visit the Serology section of this site.

( for more info click here )
Proper technique for inflation of rodent lungs for histopathologic analysis
Underinflated lung (100X) precludes accurate histologic interpretation
 Well inflated lung (100X)

Step 1. Skin animal to expose ventrum of thorax and neck.
Step 2. Cut along both sides of the ribs, remove rib cage, and dissect cervical musculature to expose trachea.
Step 3. Inspect each lung lobe for gross lesions.
Step 4. Insert needle (attached to formalin filled 20cc syringe) into trachea. Grasp trachea around inserted needle with forceps to prevent backflow of formalin.
Step 5. Push syringe plunger to inflate lungs with formalin. Lungs are adequately inflated when the formalin reaches the margins of all lung lobes and the lungs fill the chest cavity.
Step 6. Remove needle and grasp trachea with forceps to keep the lumen closed. Cut trachea proximal to forceps with scissors.
Step 7. Remove entire pluck by cutting the esophagus and aorta at the diaphragm.
Step 8. Submerse lung pluck in formalin.

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