weather contact us home
Username: 
Password: 
 

• About on-line access  • Apply for UserID

RADIL Introduces Pinworm by PCR Testing
11.19.2009

In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam.  With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results.  RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation.  In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).

Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay.  For more information and pricing, please click here. 

( for more info click here )
MFI2 - The next generation in serology testing
11.19.2009

At this year's National AALAS Meeting in Denver, Colorado, RADIL introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents.  MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available.   By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results.  Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.

For more information regarding MFI2, please visit the Serology section of this site.

( for more info click here )
Survival Blood Collection SOP

Materials Needed:

Blood collection (saphenous):

  • 50ml conical tube
  • 25G needles (5/8 inch)
  • capillary tube
  • bulb to expel blood (Globe Scientific Inc. pipette bulb cat #51675)
  • 2x2 gauze
  • small mouse clippers
  • 1.5 ml eppendorf tubes with 140microliters PBS
  • rack to hold eppendorf tubes


Blood collection (retro-orbital):

  • isoflurane anesthetic machine
  • anesthetic chamber & mask
  • capillary tube with heparin
  • bulb to expel blood (Globe Scientific Inc. pipette bulb cat #51675)
  • 2x2 gauze
  • 1.5 ml eppendorf tubes with 140microliters PBS
  • rack to hold eppendorf tubes

Blood collection technique - lateral saphenous vein

  1. Make mouse holder by poking holes in bottom of 50ml conical tube.
  2. Place mouse in conical tube with a rear leg extended outward. 
  3. Shave leg to aid in visualization of lateral saphenous vein.
  4. Use needle to prick lateral saphenous vein and allow blood to form droplet.
  5. Hold capillary tube to droplet.
  6. Once capillary tube is full, immediately add contents to Eppendorf tube with PBS using bulb to expel blood.
  7. Use gauze to apply direct pressure over lateral saphenous vein to stop bleeding.  Once bleeding has stopped move mouse back to cage.
  8. Discard empty capillary tube in sharps container and cap Eppendorf
  9. Refrigerate Eppendorf tube overnight to allow complete clotting.
  10. Centrifuge briefly if required, draw off 1:5 diluted serum* from clot.
  11. Ship on -20C ice packs to RADIL.

Blood collection technique - retro-orbital sinus

  1. Anesthetize mouse in isoflurane chamber.
  2. Remove mouse from chamber, hold mouse such that eye slightly bulges, then gently rotate capillary tube in medial canthus until blood fills capillary tube.
  3. Once capillary tube is full, immediately add contents to Eppendorf tube with PBS using bulb to expel blood, then discard capillary tube in sharps container and cap tube.
  4. Use gauze to apply direct pressure over closed eye to stop bleeding. Once bleeding has stopped move mouse back to cage and allow to recover from anesthesia.
  5. Refrigerate Eppendorf tube overnight to allow complete clotting.
  6. Centrifuge briefly if required, draw off 1:5 diluted serum* from clot.
  7. Ship on -20C ice packs to RADIL.

*Whole blood (~40% cellular by volume) diluted 1:3 yields serum diluted approximately 1:5

University of Missouri-Columbia
©2007 - Curators of the University of Missouri. All rights reserved. DMCA and other copyright information.
An equal opportunity/affirmative action institution.
design provided by Nine Plus One