11.19.2009
In the past, in order to accurately determine the presence of pinworms in an animal has required a post-mortem direct exam. With the introduction of RADIL's new PCR assay which tests for both Syphacia obvelata and Aspiculuris tetraptera, antemortem testing can now be performed with highly accurate results. RADIL's Pinworm PCR assay is nearly as sensitive as the direct exam and has the advantage that the animal does not need to be euthanized for evaluation. In studies, it was also more sensitive than either of the two antemortem tests (tape test and fecal float).
Pinworm by PCR evaluation will be available beginning December 1, 2009 as part of the Mouse Basic , Mouse Comprehensive and Rat Basic Fecal Panels, as a Helicobacter & Pinworm panel, or as a stand-alone assay. For more information and pricing, please click here.
( for more info click here )11.19.2009
At this year's National AALAS Meeting in Denver, Colorado, RADIL 
introduced a breakthrough serologic testing technology that will offer clients an increased level of results confidence for the most prevalent mouse and rat agents. MFI2 represents an advanced approach to serologic monitoring for laboratory animal pathogens, providing the highest level of diagnostic accuracy available. By evaluating multiple antigens for each agent, primary and confirmatory testing now occur at the same time, saving time and increasing the predictive value of the final results. Clients will begin seeing multiple antigens reported on case reports as of December 1, 2009.
For more information regarding MFI2, please visit the Serology section of this site.
( for more info click here )Recommended Breeding Scheme:
- Mate animals (donor) containing genetic region of interest (transgene, knock-out gene, etc.) to animals of recipient strain to produce the F1 generation. Recipient animals must be inbred and homozygous at the locus of interest. All F1 animals will be 100% heterozygous at every locus.
- Mate F1 animals to animals of recipient strain. We do not use genetic markers on the X or Y chromosomes in our assay therefore it is recommended that the sex chromosomes be fixed early in the breeding scheme for convenience. Mate female F1 animals to male recipient strain animals to fix the Y and generate the N2 generation.
- Analyze the DNA from 8-10 males who carry the genetic region of interest by microsatellite analysis. We offer genotyping services for performing or developing gene-specific PCR assays to genotype for the genetic region of interest or the client can perform the genotyping prior to sending tail snips for microsatellite analysis.
- Based on the microsatellite analysis, you choose 2-4 animals that have the greatest percentage of recipient genome and mate these to female recipient strain animals. This will generate the N3 generation and fix the X chromosome in the males.
- Analyze the DNA from 8-10 males with the genetic region of interest from the N3 generation by microsatellite analysis. Choose the 2-4 males that have the greatest percentage of recipient genome. Continue this selected breeding backcross process through the N5 generation. At this point, >99% of the genome should be derived from the recipient genetic background.
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